ABSTRACT
Neamine, a non-toxic derivative of neomycin, has recently been shown to have antitumor activities in various types of cancers. However, its effect on pancreatic cancer is still unknown. The study aimed to investigate its antitumor activity on pancreatic cancer and the underlying mechanisms. MTT assay was used to observe the effect of neamine on angiogenin (ANG)-induced AsPC-1 cell proliferation. Tissue microassay and immunofluorescence staining were used to detect the expression of ANG and its nuclear translocation, respectively. Tumor xenografts were established by subcutaneous inoculation of AsPC-1 pancreatic cancer cells into the right flanks of nude mice, and neamine was injected subcutaneously. Immunohistochemistry was done to observe the expression of ANG, CD31 and Ki-67 in tumor xenografts. It was found that neamine blocked the nuclear translocation of ANG effectively and inhibited ANG-induced AsPC-1 cell proliferation in a dose-dependent manner. Neamine had anti-tumor effects on AsPC-1 xenograft models. Consistently, neamine reduced the expression levels of ANG, Ki-67 and CD31 in tumor xenografts. It was concluded that neamine may be a promising agent for treatment of pancreatic cancer.
Subject(s)
Adult , Animals , Humans , Male , Mice , Middle Aged , Antibiotics, Antineoplastic , Pharmacology , Therapeutic Uses , Carcinoma , Drug Therapy , Cell Line, Tumor , Cell Proliferation , Framycetin , Pharmacology , Therapeutic Uses , Ki-67 Antigen , Genetics , Metabolism , Mice, Inbred BALB C , Mice, Nude , Pancreatic Neoplasms , Drug Therapy , Platelet Endothelial Cell Adhesion Molecule-1 , Genetics , Metabolism , Ribonuclease, Pancreatic , Genetics , MetabolismABSTRACT
OBJECTIVE:To investigate the stability of neamine(crude drug),a foreign material in ribostamycin sulfate by HPLC.METHODS:The separation was performed on C 18 column,the mobile phase was0.02mol/L sodium heptanesulfonate solution prepared with water-glacial acetic acid-methanol(25∶5∶70)with a flow rate of1.0ml/min and UV detection wavelength of330nm.RESULTS:The experimental data were subjected to matched t test(?=0.05),as compared to the test before,there was significant difference after experiment for neamine(the foreign material)at60℃under highlight radiation;However,no significant difference was noted at40℃under high humidity condition before and after test.CONCLUSIONS:Neamine(foreign material)in unstable at60℃under highlight radiation while stable at40℃under high humidity condition.